In addition to individual research laboratory spaces for each faculty/student research team to conduct their work, the Chemistry Department offers students research-grade instrumentation to support their education. The Neidig-Garber Science Center is a PUI facility and our instrumentation is impressive by many standards. In addition to their research application, we incorporate all instruments in the chemistry curriculum, beginning as soon as the freshman year, and through our many funded faculty/student summer research projects.
Waters Alliance HPLC-Quattro Micro Mass Spectrometer (LC-MS-MS) System. This instrument combines high performance liquid chromatography with electrospray mass spectrometry. The instrument is used to identify and quantitate trace levels of metabolites in complex cellular extracts and to determine pollutants in dilute wastestreams.
Bruker Avance 300 MHz Fourier-Transform Superconducting Broadband Nuclear Magnetic Resonance Spectrometer (FT-NMR). This instrument allows our students to determine the chemical structures of organic molecules, even very complex ones. FTNMR is the key instrument in research areas related to organic chemistry.
Bruker MicroFlex Reflectron MALDI-TOF Mass Spectrometer. This instrument, based on matrix-assisted laser desorption ionization (MALDI), is used to determine the molecular massess of intermediate to very large sized molecules. Our students use it for studying proteins, peptides, nucleic acids, inorganic complexes, and organic polymers.
Agilent 6898/5973 Gas Chromatograph-Mass Spectrometer (GC-MS). This instrument combines the powerful separating ability of capillary gas chromatography with quadrupole mass spectrometry to create an instrument that can separate the components of complex mixtures (like gasoline or perfume) and identify each of the many components found in those mixtures. This instrument is a workhorse in forensic and environmental chemistry.
Nicolet 380 Fourier-Transform Infrared Spectrometer (FTIR). This instrument measures the absorbance of infrared radiation by organic and inorganic compounds. The infrared spectrum taken by this instrument can serve as a "fingerprint" for a molecule. It is used in chemical research, forensic, and environmental chemistry.
ThermoElectron Evolution 300 Rapid Scanning Double Beam Ultraviolet-Visible Spectrophotometer with Peltier Temperature Control (UV-VIS). This spectrometer measures absorbance of light in the ultraviolet and visible ranges of the electromagnetic spectrum. It is used commonly for studying kinetics, including enzyme assays, for determining equilibrium constants, and for determining the amounts of compounds in solution.
Teledyne Isco CombiFlash RF-200 Automated Flash Chromatography System. This new preparative liquid chromatograph is a workhorse in the laboratories of Dr. Harris and Peelen. Its primary use is to purify and collect products resulting from organic synthesis.
Agilent Series 1100 Quaternary High-Performance Liquid Chromatograph with Autosampler (HPLC). This instrument is used by our students to separate, identify, and quantitate components of complex mixtures, often in aqueous solution. Used in all areas of chemistry and in biological applications.
Molecular Devices SPECTRAmax PLUS Microplate Spectrophotometer (UV-VIS). This instrument allows students in biochemistry to initiate a large number of enzyme assays in the wells of a micro plate, and the instrument subsequently follows all of the assays automatically.
Shimadzu Model RF 5301 Fluorescence Spectrophotometer. This instrument is used to study molecules that fluoresce: they emit light of a longer wavelength after being excited with light of a shorter wavelength. Fluorescence spectroscopy is often used to study compounds found at very low trace levels.
MBraun LabMaster 130 Inert Atmosphere Chamber (Glove Box). The glove box allows students to work in a chamber to prepare solutions of compounds that cannot be exposed to water or oxygen. The atmosphere within the chamber is entirely nitrogen gas which is inert and unreactive. Students work in the box by placing their arms into long gloves that reach into the chamber.
Perkin Elmer AAnalyst 100 Flame Atomic Absorption Spectrometer (FAAS). This instruments is used for the analysis of metal ions in aqueous solution. The instrument aspirates the solution into a flame where the absorbance of the metal is measured bu ultraviolet or visible light. Used often for environmental streamwater analysis of heavy metal pollutants.
Princeton Applied Research Model 273A Voltammetry/Coulometry Instrumentation (CV). This instrument uses two- and three-electrode arrangements to carry out a variety of electrochemical techniques. We use it most often for cyclic voltammetry to determine standard reduction potentials for compounds that are readily oxidized or reduced.